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pbt 1 solution  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology pbt 1 solution
    Pbt 1 Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbt+solution/Triton+X-100/pm40324675-55-21-70
    Average 96 stars, based on 11926 article reviews
    pbt 1 solution - by Bioz Stars, 2026-11
    96/100 stars

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    Incubation:

    Article Title: Biomarkers of morphine tolerance and dependence are prevented by morphine-induced endocytosis of a mutant ?-opioid receptor
    Article Snippet: .. Coronal sections (30 μm) were cut on a cryostat at −20°C, preincubated in PBT solution (0.1 M phosphate buffer + 0.2% BSA and 0.2% Triton X-100) for 30 min, blocked in 5% normal goat serum in PBT solution for another 30 min, and then incubated with rabbit anti-c- fos antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1:1000 dilution overnight at 4°C. .. Coronal sections (30 μm) were cut on a cryostat at −20°C, preincubated in PBT solution (0.1 M phosphate buffer + 0.2% BSA and 0.2% Triton X-100) for 30 min, blocked in 5% normal goat serum in PBT solution for another 30 min, and then incubated with rabbit anti-c- fos antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1:1000 dilution overnight at 4°C.

    Article Title: The monocyte locomotion inhibitory factor inhibits the expression of inflammation-induced cytokines following experimental contusion in rat tibia.
    Article Snippet: After 1 cm x 1 cm incisions were made in the injured tissue, sections were fixed with 4% paraformaldehyde for 72 h and incubated in 30% sucrose for 48 h. The sections were placed on slides treated with poly-L-lysine solution 10% (Sigma Chemical Co., St. Louis, MO, USA), incubated at A cc ep te d A rt ic le This article is protected by copyright. .. All rights reserved. room temperature for 30 min in 0.12 mol/L phosphate buffered saline Triton X-100 (PBT), pH 7.4, and then blocked with fetal bovine serum and PBT solution for 2 h. Primary antibodies goat anti-rat IL-6 (R-19):sc-1266, goat anti-rat IL-10 (A-2): sc-365858, goat anti-rat TNF-α (L-19):sc-1351, goat anti- rat TGF- (3C11):sc-130348 and goat anti-mouse IFN- (G-30);sc-57208 [ (Santa Cruz Biotech, CA, USA)] were used at a 1:800 dilution, followed by incubation for 24 h at room temperature in a humidified chamber in the dark. .. Secondary monoclonal antibodies coupled to green, yellow or red fluorochromes Alexa Fluor® 488 (mouse anti-goat IgG-CFL488:sc-516241), Alexa Fluor® 555 (mouse anti-goat IgG-CFL555:sc-516242), or Alexa Fluor® 647 (mouse anti-goat IgG-CFL647:sc-516244), respectively (Santa Cruz Biotech) were used at a 1:200 dilution, followed by incubation for 2 h. The slides were then washed with PBT solution, stained with propidium iodide, and coverslipped in Vecta-shield mounting medium (Vector Laboratories, CA, USA).

    Saline:

    Article Title: The monocyte locomotion inhibitory factor inhibits the expression of inflammation-induced cytokines following experimental contusion in rat tibia.
    Article Snippet: After 1 cm x 1 cm incisions were made in the injured tissue, sections were fixed with 4% paraformaldehyde for 72 h and incubated in 30% sucrose for 48 h. The sections were placed on slides treated with poly-L-lysine solution 10% (Sigma Chemical Co., St. Louis, MO, USA), incubated at A cc ep te d A rt ic le This article is protected by copyright. .. All rights reserved. room temperature for 30 min in 0.12 mol/L phosphate buffered saline Triton X-100 (PBT), pH 7.4, and then blocked with fetal bovine serum and PBT solution for 2 h. Primary antibodies goat anti-rat IL-6 (R-19):sc-1266, goat anti-rat IL-10 (A-2): sc-365858, goat anti-rat TNF-α (L-19):sc-1351, goat anti- rat TGF- (3C11):sc-130348 and goat anti-mouse IFN- (G-30);sc-57208 [ (Santa Cruz Biotech, CA, USA)] were used at a 1:800 dilution, followed by incubation for 24 h at room temperature in a humidified chamber in the dark. .. Secondary monoclonal antibodies coupled to green, yellow or red fluorochromes Alexa Fluor® 488 (mouse anti-goat IgG-CFL488:sc-516241), Alexa Fluor® 555 (mouse anti-goat IgG-CFL555:sc-516242), or Alexa Fluor® 647 (mouse anti-goat IgG-CFL647:sc-516244), respectively (Santa Cruz Biotech) were used at a 1:200 dilution, followed by incubation for 2 h. The slides were then washed with PBT solution, stained with propidium iodide, and coverslipped in Vecta-shield mounting medium (Vector Laboratories, CA, USA).



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